The objective of the IMPRESS project was to develop an affordable, portable, multiplexing and flexible Surface Plasmon Resonance (SPR) biosensor device (the IMPRESSOR), based on Plasmore’s Imaging Nanoplasmonics™ (iNP) technology, to obtain a fast impression of the quality and safety of food.
The three partners in the consortium had totally different but complementary expertise. The combination of a biosensor (chip) developer/producer (Plasmore) with a peptide synthesizer (Schafer-N) and a food safety control institute developing and producing antibodies against food contaminants (RIKILT) resulted in unique innovative results for the detection of food contaminants with a special focus on allergenic protein detection combined with model assays for antibiotics and mycotoxins.
The newly developed prototype is affordable (estimated production price of 10k€ (in mass production)), portable ((thick) laptop format), suitable for multiplexing (up to 1000 spots), based on Nanoplasmonic SPR in which multiple assays can be performed simultaneously within 15 min. The coated (spotted) biosensor chips are reusable for over 50 times, which reduces the costs of the consumables, and the sensitivities are in the ranges of the requirements (ppb for the mycotoxins and antibiotics and ppm for the allergens). The synthetic epitope-containing peptides (SEPs) improved the biosensor immunoassays for allergenic proteins and combined detection kits with mycotoxin and antibiotic assays could be performed which is promising for the future application.