Work Package 3

The objective of the WP was the development of high-density peptide microarrays for food allergens to find the epitopes towards the selected antibodies react.

This technology of Schafer-N was that far developed that the new goal was to focus on the application. Different high-density peptide microarrays were prepared with up to 100 000 peptides each and epitopes were found for selected commercially available polyclonal antisera and for poly- and monoclonal antibodies of RIKILT. For selected epitopes, synthetic epitope-containing peptides (SEPs) were prepared by Schafer-N and delivered to RIKILT and Plasmore for biosensor testing with very interesting results.

WP 3